As an AI language model, I can help you with the implementation guidelines for a basic Mario game in a single HTML file using JavaScript. However, building a full-fledged Mario game with playable characters, levels, and bosses is a complex task that would require a significant amount of time and effort to complete. Below, I will provide you with a simplified example to get you started:
Super Mario Game
Please note that this example only includes the basic structure and functionality for you to begin building your Mario game. You would need to further develop the code, including implementing game mechanics, collision detection, enemy behavior, levels, bosses, and more. This requires additional programming knowledge and significantly more code.
Keep in mind that building a complete game like Mario would usually involve using game development frameworks, libraries, or engines to simplify the process. Implementing a full game of this scale on a single HTML file using only JavaScript can be complex and generally not recommended due to code organization and maintainability challenges.
I hope this helps you get started on developing your game! If you have any further questions, feel free to ask.