Sure! Here's an example of a self-evolving animated pixel girl game implemented as a single HTML file using JavaScript:
When you open this HTML file in any web browser, you will see a canvas where a pixel girl is drawn. The pixel girl will continuously evolve by increasing her size every frame until she reaches a certain size and changes her color. The self-evolving animation will keep running as long as the web page is open.
Note: This is just a basic implementation to give you an idea. You can enhance this game by adding more interactive features or integrating user input to control the pixel girl's actions.